Agroecology 2 min

Copying nature to resist viruses

PRESS RELEASE - One of the impacts of climate change is the proliferation of viruses on cultivated plants. To reduce the use of pesticides and promote the deployment of agroecological practices, one strategy is to increase the natural resistance of plants to viruses. Promising results, using genome editing to mimic natural selection, were published on 30 January in the Plant Biotechnology Journal.

Published on 30 January 2023

illustration Copying nature to resist viruses
© Kyoka Kuroiwa - INRAE

For thousands of years, crops have been shaped by domestication processes. Farmers cross-breed and select new varieties, adapted to constantly changing environments. Although efficient, this process is time consuming. Moreover, the desired trait must be present somewhere within the diversity of the species to be improved.

Copying a mechanism from one species into a species of agronomic interest thus becomes a new challenge. Among the technologies available, new plant genome editing techniques have been available since 2012. Often compared to molecular scissors, the CRISPR-Cas9 technique makes it possible to modify a region of the plant's DNA in a targeted and precise manner.

Among many crop species such as pepper and pea, some cultivars are resistant to diseases caused by viruses of the genus potyvirus1 . How do they do this? To infect plants, viruses have to hijack proteins from their hosts but these proteins already fulfil functions in the plant cell. Plants that resist infection have acquired mutations in a gene that codes for one of these “susceptibility” proteins. These mutations make the use of the host protein by the virus impossible, while it remains functional for the plant. The plant with these mutations thus resists infection, without changing anything else for the plant!

A new study aimed to transpose this mechanism to plants of agronomic interest, by studying its feasibility in cherry tomatoes. To do this, the teams from INRAE used an approach that differed from the conventional approach. They did not try to inactivate the gene that makes the plant susceptible to the virus, but instead modified it to mimic the mutations responsible for resistance present in peas or peppers.


To this end, they applied the CRISPR-Cas9 technique to target two regions of this gene. These simultaneous modifications lead to changes in the protein produced by this gene. These changes give the plant a strong resistance to several viruses of the potyvirus genus, including PVY. As with natural mutations selected in other species, these changes do not alter the expression or accumulation of the protein produced by the gene, nor do they affect its function.


This work constitutes a proof of concept, proposing a new approach to editing precise mutations, aiming to reproduce natural resistances in susceptible species and thus limit the use of pesticides.


These results were obtained as part of the GENIUS project (2014-2020), supported by the French Investments for the Future programme (Programme d'Investissement d'Avenir), which aimed to develop genome editing tools to contribute to plant breeding, including pathogen resistance. A follow-up study is currently being conducted on potatoes as part of a European H2020 project GENEBECON (capturing the potential of gene editing for a sustainable bioeconomy), which aims to reduce pollution and hence climate change through the use of genome editing.

1 Potyviruses are the largest group of RNA viruses affecting plants. It encompasses several economically important viruses, including potato virus Y (PVY), which poses a threat to several plants of agronomic interest including potato, tobacco, tomato and pepper. 


Kuroiwa, K., Danilo, B., Perrot, L., Thenault, C., Veillet, F., Delacote, F., Duchateau, P., Nogue, F., Mazier, M., Gallois, J-L.
(2023) An iterative gene-editing strategy broadens eIF4E1 genetic diversity in Solanum lycopersicum and generates resistance to multiple potyvirus isolates. Plant Biotechnol J.,

Learn more

Society and regional strategies

Ethics and politics of plant-genome editing: joint-committee report and position

Genome editing and its use as a tool to develop new plant species present ethical and political challenges that have been little discussed until now. Opinion 11 from the joint INRA-CIRAD-IFREMER Ethics Advisory Committee turns a powerful lens on the intricacies of these questions, analysing the value systems and symbolic representations that underlie the wide-ranging issues it identifies. Its discussions focus on the example of the CRISPR-Cas9 genome-editing system.

23 March 2018


Thierry Candresse, exploring the nanoworld of viruses

An ardent observer of nature, fruit-tree specialist and plant virome pioneer, Thierry Candresse is an internationally recognised virologist. Thierry is a research director at the INRAE Nouvelle-Aquitaine-Bordeaux centre, dedicating himself to research, research management, teaching and supporting agricultural stakeholders. He is the recipient of the 2022 Lifetime Achievement Award.

29 November 2022


Plant genome editing: INRA defines its strategy

PRESS RELEASE - Plant genome editing is a technology that attracts much social and regulatory debate: INRA has defined a strategy for the use of these new techniques to acquire new knowledge and in plant breeding. The principles underlying this strategy are in line with the Institute’s values and with its ambition to contribute to environmental, social and economic progress. INRA developed its strategy based on the recommendations of an opinion issued by the Joint INRA-CIRAD-IFREMER Ethics Advisory Committee in the spring of 2018, along with the opinion of its Scientific Advisory Board, issued last September.

23 April 2020