Agroecology 3 min

Training sheep to complete awake MRI imaging

PRESS RELEASE - Sheep are capable of learning a wide range of complex tasks. A research team from INRAE was able to prove this by training sheep to undergo MRI scans while awake—a world-first. This innovative method, which hinges on trainer–lamb cooperation, helps produce quality images without the need for anaesthesia. The results, published in Behavior Research Methods, could offer new insights into how sheep’s brains work. The study could also lead to new applications in farm animal veterinary care.

Published on 01 July 2024

illustration Training sheep to complete awake MRI imaging
© INRAE- Sophie Normant

MRI or magnetic resonance imaging is a technique commonly used to explore the brains of sheep. Until now, it had only been performed under general anaesthesia, to ensure the animal’s immobility. Anaesthesia, however, leads to stress and other negative side-effects, in addition to jeopardising the study of brain activity.

A research team from INRAE has developed a training protocol adapted to sheep in order to carry out MRI acquisitions in awake animals, without the need for restraint. To do this, researchers drew on previous work with dogs, which until now had been the only animal species capable of carrying out this type of protocol. In the nursery of the Animal Physiology Facility experimental research unit (UEPAO), located at the INRAE Val de Loire centre in Nouzilly, researchers began a familiarisation phase as soon as the lambs were born. The objective was to identify which animals were most receptive to being stroked or to having foam objects placed near their heads.

After choosing 10 lambs, an initial training phase took place at the Nouzilly sheep farm. The research team trained the animals to climb a ramp to reach a mock MRI scanner and then lie down. The lambs were also taught to place their heads in a mock MRI coil.

Once they arrived at the real MRI room, the sheep were able to reproduce the same behaviour very easily, but had some difficulty remaining perfectly still. It took a few weeks for the animals to get used to the vibrations of the machine and stop moving for a few minutes. Ultimately, the MRI images of their brains were comparable to those obtained from anaesthetised sheep, a goal that was initially achieved in 6 out of the 10 sheep trained at the time of writing, and has since been achieved in 9 sheep. The protocol lasted 9 months, from the birth of the lambs to the first MRI acquisitions.

Chronology of stages leading up to the image acquisition phase. INRAE - Love and Pluchot

The success of this protocol is already opening up new avenues for research into animal neuroimaging (e.g. fMRI)—since it makes it possible to study brain function in awake animals. A study looking into the activation of certain brain regions in relation to hearing is currently underway, and is the subject of a PhD thesis that relies on this training protocol. This example of voluntary cooperation between trainer and sheep illustrates the animal's ability to learn, and underlines the importance of human-animal relationships in the development of innovative methods. The study also opens up new possibilities for training other animals to carry out awake MRI scans. Such training methods could have numerous other applications, in areas such as shearing or medical training—when the animal learns to collaborate during veterinary care.


Pluchot C., Adriaensen H., Parias C. et al. (2024). Sheep (Ovis aries) Training Protocol for Voluntary Awake and Unrestrained Structural Brain MRI Acquisitions. Behavior Research Methods, DOI:

PR_sheep_MRI_ENGpdf - 338.64 KB



Camille Pluchot Reproductive Physiology and Behavior Joint Research Unit

Scott Love Reproductive Physiology and Behavior Joint Research Unit



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